CYTOPHOTOMETRIC DETERMINATION OF PROTEIN-BOUND SULFHYDRYL GROUPS
نویسندگان
چکیده
منابع مشابه
Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.
Studies of protein-bound (PB-SH) and nonprotein bound sulfhydryl group (NP-SH) concentrations in tissues under various conditions is a prerequisite to understanding the role of sulfhydryls in living organisms. Many methods have been developed for the measurement of sulfhydryl groups, but these have been concerned chiefly only with the estimation of NP-SH in biological fluids or total SH groups ...
متن کاملDetermination of Protein-bound Iodine*
The growing interest in chemical evaluation of thyroid function by use of the plasma level of protein-bound iodine (PI) is attested by the increasing numbers of reports in which this criterion is employed, both chemically and experimentally. However, the specific techniques available for such a determination do not yet seem sufficiently reliable. The purpose of this communication is to describe...
متن کاملDetermination of Protein-bound Iodine*
The growing interest in chemical evaluation of thyroid function by use of the plasma level of protein-bound iodine (PI) is attested by the increasing numbers of reports in which this criterion is employed, both chemically and experimentally. However, the specific techniques available for such a determination do not yet seem sufficiently reliable. The purpose of this communication is to describe...
متن کاملSulfhydryl Groups in Proteins
Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...
متن کاملDetermination of protein-bound glutamine and asparagine.
The correct assignment of amide groups to the glutamyl and aspartyl residues determined from analyses of acid hydrolysates continues to be an important problem in the delineation of protein and peptide structure. This problem is illustrated by recent discussions by Potts et al. (1) and Smyth, Stein, and Moore (2) on the elucidation of ribonuclease structure and by Chibnall et al. (3) on the est...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1955
ISSN: 0022-1554,1551-5044
DOI: 10.1177/3.5.354